Clémence Poésy (Mary, Queen of Scots), Kevin McKidd (Bothwell), Michael Fassbender (Guy Fawkes), Emilia Fox (Lady Margaret), Tim McInnerny (Cecil), Catherine McCormack (Queen Elizabeth I), Gary Lewis (John Knox), Paul Nicholls (Lord Darnley), Carmen Ungureanu (Mary of Guise), Tadeusz Pasternak (David Riccio), Maria Popistasu (Lady Marie), Steven Duffy (Lord James), Catalin Babliuc (Father Michael), Emil Hostina (Bothwell's Lieutenant), Daniela Nardini (Lady Huntly), Radu Andrei Micu (Sir John Huntly), Iona Ruxandra Bratosin (Young Mary), Garry Sweeney (Lord Gunn), Michael Nardone (Lord Ruthven), Adrian Stefan (Taylor), Sira Stampe (Queen Anne of Denmark), Iain Robertson (James Huntly), Richard Coyle (Catesby), Richard Harrington (Thomas Percy), Sam Troughton (Thomas Winter), Patrick O'Kane (Francis Tresham), Julian Firth (Peers The Parliamentarian)